Application | Comment | Organism |
---|---|---|
food industry | mutant enzyme Y169C/A211C is a good candidate for the bread baking industry. It has a significant effect on bread volume. Its improved thermostability, pH stability, and catalytic activity, its resistance to SDS, maximal enzymatic activity at low temperatures, and high activity under alkaline conditions are valuable properties that in addition to the bread baking industry | Cladosporium neopsychrotolerans |
Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Cladosporium neopsychrotolerans |
Protein Variants | Comment | Organism |
---|---|---|
Y169C/A211C | compared with wild-type enzyme, the half-life of the mutant, at 40 °C increases approximately 48fold. The kcat and catalytic efficiency are enhanced 0.7fold and 1.6fold, respectively | Cladosporium neopsychrotolerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 10 mM, wild-type enzyme retains approximately 80% of its activity, mutant enzyme Y169C/A211C retains 50-60% activity | Cladosporium neopsychrotolerans | |
Mn2+ | 5 mM and 10 mM, enzymatic activity of wild-type enzyme is above 100%, mutant enzyme Y169C/A211C retains 50-60% activity | Cladosporium neopsychrotolerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
69 | - |
beta-D-glucose | pH 7.0, 20-25°C, mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans | |
103 | - |
beta-D-glucose | pH 7.0, 20-25°C, wild-type enzyme | Cladosporium neopsychrotolerans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 5 mM and 10 mM, enzymatic activity of wild-type enzyme is above 100% | Cladosporium neopsychrotolerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64600 | - |
calculated from sequence | Cladosporium neopsychrotolerans |
120000 | - |
SDS-PAGE, wild-type and mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
Organic Solvent | Comment | Organism |
---|---|---|
SDS | wild-type and mutant enzyme Y169C/A211C are highle resistant | Cladosporium neopsychrotolerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cladosporium neopsychrotolerans | A0A5Q2UVJ5 | - |
- |
Cladosporium neopsychrotolerans SL16 | A0A5Q2UVJ5 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 13 putative N-glycosylation sites | Cladosporium neopsychrotolerans |
Purification (Comment) | Organism |
---|---|
- |
Cladosporium neopsychrotolerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucose + O2 | - |
Cladosporium neopsychrotolerans | D-glucono-1,5-lactone + H2O2 | - |
? | |
beta-D-glucose + O2 | - |
Cladosporium neopsychrotolerans SL16 | D-glucono-1,5-lactone + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE, wild-type and mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
Synonyms | Comment | Organism |
---|---|---|
CngoxA | - |
Cladosporium neopsychrotolerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
recombinant wild-type enzyme | Cladosporium neopsychrotolerans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
40 | 0°C: about 70% of maximal activity, 40°C: about 80% of maximal activity, recombinant wild-type enzyme | Cladosporium neopsychrotolerans |
- |
50 | 0°C: about 75% of maximal activity, 40°C: about 75% of maximal activity, mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type and mutant enzyme Y169C/A211C are cold adapted | Cladosporium neopsychrotolerans |
30 | - |
1 h, 10% loss of activity, recombinant enzyme | Cladosporium neopsychrotolerans |
40 | - |
1 h, no loss of activity, mutant enzyme Y169C/A211C. Half-life of mutant enzyme Y169C/A211C: 720 min. Half-life of wild-type enzyme: 15 min | Cladosporium neopsychrotolerans |
45 | - |
1 h, complete loss of activity, recombinant enzyme. 30 min, 60% loss of activity, mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
50.6 | - |
Tm-value of wild-type enzyme: 50.6°C | Cladosporium neopsychrotolerans |
58.6 | - |
Tm-value of mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
97 | - |
beta-D-glucose | pH 7.0, 20-25°C, wild-type enzyme | Cladosporium neopsychrotolerans | |
164 | - |
beta-D-glucose | pH 7.0, 20-25°C, mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
pH 7.0, 20-25°C, recombinant wild-type enzyme | Cladosporium neopsychrotolerans |
7.5 | - |
pH 7.0, 20-25°C, mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0: about 60% of maximal activity, pH 10.0: about 40% of maximal activity, recombinant wild-type enzyme | Cladosporium neopsychrotolerans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 12 | 1 h, stable, mutant enzyme Y169C/A211C | Cladosporium neopsychrotolerans |
6 | 9 | 1 h, stable, recombinant enzyme | Cladosporium neopsychrotolerans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cladosporium neopsychrotolerans | calculated from sequence | - |
4.66 |